Primique | Tool name | Primique |
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| URL | http://cgi-www.cs.au.dk/cgi-chili/primique/front.py |
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| Important features | Designs primer pairs specific to the family of closely related species. |
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| Citations | Fredslund J, Lange M. Primique: automatic design of specific PCR primers for
each sequence in a family. BMC Bioinformatics. 2007 Oct 3;8:369. PubMed PMID:
17910777; PubMed Central PMCID: PMC2045118. |
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| Year of publication | 2007 |
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| Rank by usage frequency | 100 |
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| Comments | |
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| Function | Phylogenetic analysis, General PCR, Primer specificity checking, Predicting secondary structure |
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| Category | Online, Free |
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| Features | 1. Designs PCR primers and specific pair of primers for each sequence in a family of closely related and very similar sequences.
2. Export option is available. Option to check the specificity of primer [i.e. Blast the primer against NCBI sequence to check the specificity of primers against the non-target sequence]
3. Option to specify general primer parameters such as primer length range, Tm range, GC%, etc.
Note: Tool lists only the best primers and not all the possible primers. |
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| Description | |
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