| Tool name | Primers4clades |
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| URL | http://maya.ccg.unam.mx/primers4clades/ http://floresta.eead.csic.es/primers4clades/ |
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| Important features | 1. Designs PCR primers for cross-species amplification of novel sequencesA0from metagenomic DNA and from uncharacterized organisms belonging to user defined phylogenetic clades or taxa.
2. Useful for phylogenetic analysis. |
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| Citations | Contreras-Moreira B, Sachman-Ruiz B, Figueroa-Palacios I, Vinuesa P.
primers4clades: a web server that uses phylogenetic trees to design
lineage-specific PCR primers for metagenomic and diversity studies. Nucleic Acids
Res. 2009 Jul 1;37(Web Server issue):W95-W100. Epub 2009 May 21. PubMed PMID:
19465390. |
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| Year of publication | 2009 |
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| Rank by usage frequency | 100 |
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| Comments | Features are written as claimed by the author. Not validated completely. |
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| Function | Phylogenetic analysis, Degenerate primers, General PCR |
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| Category | Online/downloadable, Free |
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| Features | 1) PCR primers for cross-species amplification of novel sequencesA0from metagenomic DNA and from uncharacterized organisms belonging to use defined phylogenetic clades or taxa.
2) Uses phylogenetic trees to design lineage-specific PCR primers for metagenomic and diversity studies.
3) Multiple alignment of the sequences and suggests degenerate primers to amplify homologous regions
4) Useful for phylogenetic analysis.
Note: Comment line version is available. |
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| Description | |
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