Conserved Primers 2.0 | Tool name | Conserved Primers 2.0 |
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| URL | http://probes.pw.usda.gov/cgi-bin/ConservedPrimers/ConservedPrimers.cgi |
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| Important features | Designs intron flanking primers [i.e. primers that span the intron sequence] for the plants such as Oriyza sativa, Sorghum bicolour, Barchypodium distachyon, Arabindopsis thaliana, Glycine max etc. |
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| Citations | You FM, Huo N, Gu YQ, Lazo GR, Dvorak J, Anderson OD. Conserved Primers 2.0: a
high-throughput pipeline for comparative genome referenced intron-flanking PCR
primer design and its application in wheat SNP discovery. BMC Bioinformatics.
2009 Oct 13;10:331. PubMed PMID: 19825183. |
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| Year of publication | 2009 |
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| Rank by usage frequency | 100 |
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| Comments | |
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| Function | SNP primers |
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| Category | Online, Free |
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| Features | 1. Accepts only the EST sequences as an input.
2. Blast EST sequence against the reference genome to identify the intron sequence and intron flanking sequence. Option to specify the E-value and maximum intron length to obtain. |
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| Description | For amplifying multiple targets in a single reaction. |
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