WMD3- Web microRNA designer | Tool name | WMD3- Web microRNA designer |
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| URL | http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Home;project=stdwmd |
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| Important features | Web based tool that was developed to assist a user in the designing of artificial microRNAs. |
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| Citations | Ossowski S, Schwab R, Weigel D. Gene silencing in plants using artificial
microRNAs and other small RNAs. Plant J. 2008 Feb;53(4):674-90. Review. PubMed
PMID: 18269576 |
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| Year of publication | 2008 |
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| Rank by usage frequency | 100 |
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| Comments | Open access ( GNU General Public License (GPL) ) |
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| Function | EXPERIMENTAL aids; RNA interference, SPECIES: plant, http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Home;project=stdwmd, Web based tool that was developed to assist a user in the designing of artificial microRNAs., Ossowski S- Schwab R- Weigel D. Gene silencing in plants using artificial
microRNAs and other small RNAs. Plant J. 2008 Feb;53(4):674-90. Review. PubMed
PMID: 18269576, 2008, 100, Open access ( GNU General Public License (GPL) ), Online/ Downloadable, I. SPECIES SUPPORTED INCLUDE: Plant II. QUERY OR INPUT DATA: 1. Target search: enter microRNA sequence and select genome from the given list- other options include microRNA filter and advanced search options 2. Designer: Enter target genes- select genome and minimum number of included targets- description and Email ID 3. Oligo: Enter microRNA sequence and choose vector 4. Hybridize: Enter two sequences of interest and temperature 5. BLAST: Enter sequence in FASTA format and select genome from the given list III. OUTPUT DATA: details on possible candidates., Rebecca Schwab- Stephan Ossowski- Markus Riester- Norman Warthmann- and Detlef Weigel. (2006) Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis Plant Cell 18: 1121-1133 |
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